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Nonsanto The future of farming! Founded by: Dr. D. A. Possum

Founded in 1973, Nonsanto’s original goal was to use recombinant DNA technology to form an insulin-producing bacteria. Our lead scientists modified bacteria from the common cold, removed the genetic material responsible for many of the symptoms and added the insulin-producing gene. Under the protocol, patients would receive a once-yearly injection into the pancreatic duct, and the bacteria injected would act like a film, coating the duct and producing insulin. In spite of some successful initial outcomes in experiments using rats, we saw no major improvement in human insulin production. After that setback, we applied the data we amassed to improving staple food production, specifically corn. We are currently developing a cold-resistant crop.
 * About Us**

I (Dr. D.A. Possum) founded our company with little more than a plan and my parents’ retirement money. Introduced to this technology by my 9th grade biology teacher, I continued my studies at University of California at San Francisco, where new developments in genetics were being explored, specifically the vast potential of combining the traits of separate organisms. Since 1982, I’ve directed my efforts exclusively to what is now called recombinant DNA technology.
 * Our Founder **

One of the forerunners of using recombinant DNA technology is a company named Genentech, started as the brain child of Herbert Boyer in 1976. Previously, Herbert Boyer had worked with another scientist, Stanley Cohen, to conduct the first successful recombinant DNA experiment in 1972. In 1978, they produced the first-ever human protein using recombinant DNA, Somatostain (which regulates human growth hormones). These pioneers in genetic technology are the examples I used to base my company and I will strive to make as many important contributions to the field as they have.
 * History of Recombinant DNA **

There are three forms of Recombinant DNA (rDNA). They are Transformation, Non-Bacterial Transformation and Phage Introduction. Transformation takes place in three steps: 1.Select the DNA that is going to be inserted. 2.Remove the chosen DNA from the strand using a restriction enzyme and then use DNA ligase to insert the DNA into the vector (such as a plasmid). The inserted DNA often has a marker such as an immunity to an anti-biotic or a change in color. 3.Insert the vector into a host cell such as E. coli, a commonly used bacteria. Non-bacterial transformation has the same first two steps as transformation, but insertion into the cell is different. The host cell in non-bacterial transformation is also not a bacteria (thus the name). The host cell is bombarded with DNA-coated particles of gold or tungsten in a process called biolistics, then the DNA is inserted directly into the nucleus of a cell in a process called microinjection. Phage introduction is the same as transformation except the target cell is a phage and not a bacteria. This process is called transfection. Phages are structures that latch onto bacteria and insert DNA. Recombinant DNA technology is being used for many things, such as: One of the most largely produced crops in America is corn, and as any self-respecting company would want, we want a monopoly. Most corn is farmed in northwestern states (see map) with a significant fallow winter period. Our current efforts are to extract the cold-weather resistance gene from an Evergreen tree and insert into a hardy strain of corn to produce a crop that could, not only be grown in colder environments but year-round, increasing the availability of inexpensive food and fuel. If we are to succeed Nonsanto will become a new farming empire! We may combine the ability to grow year-round and the ability to have the corn produce it’s own insecticides at a price now farmer could resist!
 * How Recombinant DNA Technology Works **
 * Transformation **
 * Non-Bacterial Transformation **
 * Phage Introduction **
 * Current Uses **
 * Heat and drought resistant crops
 * Recombinant vaccines such as Hepatitis B
 * Prevention and cure of diseases such as sickle cell anemia and cystic fibrosis
 * Production of insulin and recombinant pharmaceuticals
 * Insecticide producing plants
 * Somatic gene therapy.
 * Nonsanto’s Plans for the World’s Future **





"An Introduction to Recombinant DNA." Rensselaer Polytechnic Institute (RPI) :: Architecture, Business, Engineering, IT, Humanities, Science. N.p., n.d. Web. 23 Jan. 2012. < [] >. "Insertion of Foreign Genes and Vectors." VT.edu. N.p., n.d. Web. 23 Jan. 2012. . Memorial University. N.p., n.d. Web. 23 Jan. 2012. < [|www.mun.ca/biology/scarr/Fig15-14_DNA_transformation.gif] >. "Milestones in DNA History." Access Excellence. N.p., n.d. Web. 22 Jan. 2012. < [|www.accessexcellence.org/RC/AB/WYW/wkbooks/SFTS/sidebarmilestone.php] >. "Monsanto Corporation's global vegetable seed market share - Maps and Graphics at UNEP/GRID-Arendal." Maps and Graphics at UNEP/GRID-Arendal. N.p., n.d. Web. 23 Jan. 2012. < [] >. NASS. NASS. N.p., n.d. Web. 22 Jan. 2012. < [|www.nass.usda.gov/Charts_and_Maps/Crops_County/pdf/CR-PR10-RGBChor.pdf] >. NCBI. "Diabetes - PubMed Health." National Center for Biotechnology Information. N.p., n.d. Web. 22 Jan. 2012. < [] >. "The Pancreas: Anatomy and Functions ." The Ohio State University Medical Center. N.p., n.d. Web. 22 Jan. 2012. < [] >. UT SouthWestern. N.p., n.d. Web. 22 Feb. 2012. < [|www.utsouthwestern.edu/vgn/images/portal/cit_56417/40/39/178088histbiotech.pdf] >.
 * References of Information **

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