Kristin+Hartlage


 * r **** egaltrah **** laboratories **


 * About Us: **

James Hobbs is the founder Regaltrah laboratories. He started the company in 1985, with two of his colleagues Matthew Tanks and Thomas Carter. The co mpany started out small and has expanded to be one of the largest tissue culturing companies in the United States. Since the start Regaltrah laboratories has been working in the tissue culturing industry, we have had a very successful past few years and have expanded our laboratories internationally. Now we have labs in The United Kingdom, and in South Africa. We employ some of the nations top research scientists, and our company continues to grow in research and international investments.


 * Who I Am: **

I am one of the biology researchers in Regaltrah laboratories. I start edworking here in 2001 and have been helping my coworkers advance our knowledge of the tissue culturing research. I am part of a specialized team of scientists that is creating experiments on new plants to see how fast we can grow these plants. I wen t to The University of Rochester and studied Molecular biology for four years and then I went to get my Ph.D. in molecular biology at Harvard University. From there I was hired at Regaltrah laboratories as a part time researcher and have built myself up to being one of the best molecular biologist in the nation.


 * History of our technology: **

Tissue Culturing started back in 1902 when a gentleman named Haberlandt reported that he had cultured an isolated single palisade cell from leaves in a specialized Knop’s salt solution with sucrose. Haberlandt’s cell stayed alive for a month the cell grew, but failed to divide. During this month Haberlandt watched and studied the cell and came up with developmental techniques for culturing plant cells under better conditions. Finally in the third and fourth decades of the twentieth century RJ Gautheret of France and P.R. White from the U.S.A. made Harberlandt’s ideas possible. Skoog and coworkers enhanced Harberlandt’s ideas and created the most modern tissue culture media in the 1950’s and 60’s. Maheshwari and Guha in 1964 were able to take haploid plants from pollen grains and culture anthers of Datura. Now over time the techniques of protoplast production have been refined and it is now possible to regenerate whole plants from protoplasts. Also in 1972 Carlson and coworkers produced the first somatic hybrid plant by fusing the protoplasts of plants together.


 * Science behind our technology: **

The tissue culturing industry is an efficient and useful process, which allows rapid production of a genetically identical plant in a relatively small space, without a lot of supplies and time. T issue culturing techniques require taking a small part of the plant, such as the seed or stem tip, that you want to culture and grow. You put it in a sterile nutrient medium where it will multiply. The nutrient medium will vary depending on the type of plant you are using, and if you’re trying to produce embryos for artificial seeds, multiply the number of plantlets, or grow roots. Tissue culturing varies in level of difficulties by how many plants you want to grow and what you’re planning to use the plants for.

The current use of tissue culturing technology is that we can co mbine plants to make hybrid plants, the benefits to doing this is that the plants created this way are more resistant to disease and they last longer. They can also withstand climate conditions. Another use of tissue culturing is that we can create new plants with the tissue of a plant. If you have a tissue of one plant you can replicate it under the right conditions and with the right amount of plant food, plants can be grown in laboratories.
 * Current use of our technology: **

The future use of the tissue culturing technology is that we can help peopl e in different countries; we can culture the plants that we grow here in our laboratories and ship our healthy plants to places without good food sources. This would make the peoples diets healthier, safer and the food production can increase. This would happen because we would be starting with healthier plants without any diseases. Because the plants are virus and disease fre e hopefully the amount of pesticides would decrease. This makes all plant production better and the world would be a healthier place to live. Also in the future tissue culturing could not only be used in crops but also we could take tissue samples of trees and other plants in the rain forests. This way if the rain forests are ever completely destroyed, we could rebuild them faster and more efficiently. This would also make sure many of the plants in the forest don’t become extinct. They wouldn’t go extinct because we would have samples of the tissues so we could always culture them and make more seedlings.
 * Future use of our technology: **

"Plant Tissue Culture." //Access Excellence @ the National Health Museum//. Web. 22 Jan. 2012. .
 * Resources: **

The Science of Hybrid Crops." //The Wessels Living History Farm, the Story of Agricultural Innovation//. Web. 22 Jan. 2012. .

"History of Tissue Culture Techniques,Haberlandt,Embryo Culture,Laibach,Maheshwari and Guha,Pollen Culture,Haploid Plants,Auxin-Cytokinin Ratio." //Molecular Plant Biotechnology Techniques,Methods,Applications,Procedures,Genes,DNA Replication and Repair, Animal Biotechnology//. Web. 22 Jan. 2012. .

"Tissue Culture." //HORT 201 & 202 Home Page//. Web. 22 Jan. 2012. .

"History of Tissue Culture Techniques | Plant Pedia." //Plant Pedia | Plant Biotechnology | Plant Science | Plant Tissue Culture//. Web. 22 Jan. 2012. .

“Role of tissue culture in agriculturaldiversification.” Food and Agricultural Research Council.Web.22.jan.2012. 

"Tissue Culture” Available: .

// “Plant Tissue Culture” Available: < // http://www.ppm4plant-tc.com/>.

// “Daylily Tissue Culture” // Available: .